Article
Improvement of expression level of keratinase Sfp2 from Streptomyces fradiae by site-directed mutagenesis of its N-terminal pro-sequence.
Biotechnology letters - 1 May 2013
Li Junxia, Chen Dongdong, Yu Zhanqiao, Zhao Longmei, Zhang Rijun
Abstract excerpt
The keratinase Sfp2, produced by Streptomyces fradiae var. k11, is a serine alkaline protease first synthesized as pre-pro-mature precursor, of which the N-terminal propeptide must be autocatalytically cleaved on the C-terminal of P1 amino acid to produce mature enzyme. Single amino acid substitutions were introduced at positions -1 and -2 to improve the expression level of mature Sfp2. The specific activity of...
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