Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris.

PloS one - 1 Jan 2013

Hu Hong, Gao Jie, He Jun, Yu Bing, Zheng Ping, Huang Zhiqing, Mao Xiangbing, Yu Jie, Han Guoquan, Chen Daiwen

Abstract excerpt

The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced into keratinase...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacillus
Share to Gene Dosage
Share to Gene Expression Regulation, Enzymologic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Glycosylation

Share to Hydrolysis

Share to Keratins

Share to Peptide Hydrolases

Share to Pichia

Source

Open in PubMed Open full text

Identifiers

PMID: 23472192
DOI: 10.1371/journal.pone.0058393
PMCID: PMC3589435

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris.PMID 23472192Read ↗

Improvement of expression level of keratinase Sfp2 from Streptomyces fradiae by site-directed mutagenesis of its N-terminal pro-sequence.Semantic proximity Read ↗

Engineering of Komagataella phaffii for efficient secretory expression of highly hydrophilic recombinant type III human-like collagenSemantic proximity Read ↗

High-level Expression and Integrated Purification of Recombinant Human Type I+III Collagen in Pichia pastorisSemantic proximity Read ↗

The molecular modification, expression, and the antibacterial effects studies of human lysozyme.Semantic proximity Read ↗

Recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

Genotyping of recombinant Pichia pastoris strains.Semantic proximity Read ↗

Mutations in the regulatory regions result in increased streptomycin resistance and keratinase synthesis in Bacillus thuringiensis.Semantic proximity Read ↗

Cloning, characterization, and functional analysis of the EPG1-2 gene: a new allele coding for an endopolygalacturonase in Kluyveromyces marxianus.Semantic proximity Read ↗

[Construction of high-quality gene mutant pool in Pichia pastoris by a PCR dependent method].Semantic proximity Read ↗

Insulin glargine production: Codon engineering in Pichia pastoris X-33 for enhanced therapeutic yieldSemantic proximity Read ↗

The inhibition of aggregation of recombinant human consensus interferon-alpha mutant during Pichia pastoris fermentation.Semantic proximity Read ↗

High-level secretion of a chimeric thermostable lichenase from Bacillus subtilis by screening of site-mutated signal peptides with structural alterations.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.