Article
An alternative role of FoF1-ATP synthase in Escherichia coli: synthesis of thiamine triphosphate.
Scientific reports - 1 Jan 2013
Gigliobianco Tiziana, Gangolf Marjorie, Lakaye Bernard, Pirson Bastien, von Ballmoos Christoph, Wins Pierre, Bettendorff Lucien
Abstract excerpt
In E. coli, thiamine triphosphate (ThTP), a putative signaling molecule, transiently accumulates in response to amino acid starvation. This accumulation requires the presence of an energy substrate yielding pyruvate. Here we show that in intact bacteria ThTP is synthesized from free thiamine diphosphate (ThDP) and P(i), the reaction being energized by the proton-motive force (Δp) generated by the respiratory...
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