Article
Isolation by polymerase chain reaction of a cDNA whose product partially complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells.
Gene - 15 Mar 1990
Teitz T, Penner M, Eli D, Stark M, Bakhanashvili M, Naiman T, Canaani D
Abstract excerpt
A xeroderma pigmentosum (XP) cell line from complementation group C has been complemented to attain ultraviolet (UV) resistance and DNA repair proficiency, by transfection with a human expression cDNA library, followed by selection to UV resistance. We now show that the transfected cDNAs can be rescued from cellular DNA of a secondary transformant by its in vitro amplification using expression-vector-specific...
Topics
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA
- DNA Repair
- Genetic Complementation Test
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- Transfection