Article
Functional significance of loops in the receptor binding domain of Bacillus thuringiensis CryIIIA delta-endotoxin.
Journal of molecular biology - 2 Feb 1996
Wu S J, Dean D H
Abstract excerpt
Analysis of the three surface loops in domain II of Bacillus thuringiensis CryIIIA delta-endotoxin has been carried out to assess their role in receptor binding and toxicity. Site-directed mutagenesis was used to convert loop residues to alanine and the mutant proteins were analyzed for structura...
Topics
- Animals
- Bacillus thuringiensis Toxins
- Bacterial Proteins
- Bacterial Toxins
- Base Sequence
- Binding Sites
- Coleoptera
- DNA Primers
- Endotoxins
- Escherichia coli
- Hemolysin Proteins
- Insect Proteins
- Microvilli
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Receptors, Cell Surface
- Receptors, Immunologic