Article
The sim gene of Escherichia coli phage P1: nucleotide sequence and purification of the processed protein.
Virology - 1 Apr 1990
Maillou J, Dreiseikelmann B
Abstract excerpt
The sim gene of bacteriophage P1 causes exclusion of a superinfecting P1 phage. We determined the nucleotide sequence of a 1.9-kb DNA fragment that, in plasmids, causes Sim phenotype. There are two open reading frames within this region for proteins of 82 and 259 amino acids. A 1.3-kb fragment containing the larger open reading frame was inserted into an expression vector. Induced cells carrying the hybrid...
Topics
- Amino Acid Sequence
- Base Sequence
- Coliphages
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Genes, Viral
- Molecular Sequence Data
- Molecular Weight
- Phenotype
- Plasmids
- Viral Proteins