Article
Genome organization of membrane-containing bacteriophage PRD1.
Virology - 1 Aug 1991
Bamford J K, Hänninen A L, Pakula T M, Ojala P M, Kalkkinen N, Frilander M, Bamford D H
Abstract excerpt
We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage...
Topics
- Amino Acid Sequence
- Bacteriophages
- Base Sequence
- Cloning, Molecular
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Genes, Viral
- Genetic Complementation Test
- Gram-Negative Bacteria
- Molecular Sequence Data
- Mutation