Article
Fetal RhD genotyping by real time quantitative PCR in maternal plasma of RhD-negative pregnant women from the Sahel of Tunisia.
Annales de biologie clinique - 1 Jan 2000
Moussa Hajer, Tsochandaridis Marthe, Jemni-Yacoub Saloua, Hmida Slama, Khairi Hédi, Gabert Jean, Levy-Mozziconacci Annie
Abstract excerpt
OBJECTIVE: The aim of this study was to evaluate the diagnostic value of RhD fetal genotyping from the plasma of RhD-negative pregnant women. METHODS: We analysed the plasma samples of 65 pregnant women. DNA quantification was done using real time quantitative PCR (RQ-PCR) in multiplex targeting multiple RhD exons 5, 7 and 10, with a standardized pool of plasmid calibrators. Results were compared with serological...
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