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Article

Kinetic analysis of intracellular Hoechst 33342--DNA interactions by flow cytometry: misinterpretation of side population status?

Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Jan 2013

Smith Paul J, Wiltshire Marie, Chappell Sally C, Cosentino Laura, Burns Philip A, Pors Klaus, Errington Rachel J

Abstract excerpt

We outline a simple approach involving instrument setup and calibration for the analysis of Hoechst dye 33342-loading in human cell lines for exploring heterogeneity in dye efflux efficiency and the status of side population (SP) A549 lung cancer cells. Dual excitation 488 nm/multiline UV (351-364 nm) flow cytometry was used to confirm ABCG2-specific inhibition of dye efflux using Fumitremorgin C. Transporter...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to ATP Binding Cassette Transporter, Subfamily G, Member 2

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Source

Identifiers

PMID: 23136081
DOI: 10.1002/cyto.a.22224

Genes

ABCG2
ABCB1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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