Article
Construction of an h+S strain of Schizosaccharomyces pombe.
Current genetics - 1 Jan 1990
Heim L
Abstract excerpt
By spontaneous in vivo integration of a mat2:1 degrees plasmid, containing a Plus (P) cassette, into an h-L MT region of Schizosaccharomyces pombe an h+ strain was obtained which neither mutates to h- nor to h90. Southern blotting showed that it possesses the same mating-type (MT) configuration a...
Topics
- Blotting, Southern
- Crosses, Genetic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genes, Mating Type, Fungal
- Mutation
- Plasmids
- Polyploidy
- Recombination, Genetic
- Restriction Mapping
- Saccharomycetales
- Schizosaccharomyces
