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Article

Engineering Candida albicans glucosamine-6-phosphate synthase for efficient enzyme purification.

Journal of molecular recognition : JMR - 1 Nov 2012

Czarnecka Justyna, Kwiatkowska Karolina, Gabriel Iwona, Wojciechowski Marek, Milewski Sławomir

Abstract excerpt

Rationally designed muteins of Candida albicans glucosamine-6-phosphate synthase, an enzyme known as a promising target for antifungal chemotherapy, were constructed, overexpressed in Escherichia coli and purified to near homogeneity. To facilitate and to optimize the purification of the enzyme, three recombinant versions containing internal oligoHis fragments were constructed: (i) by substituting residues...

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PMID: 23108616
DOI: 10.1002/jmr.2175

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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