Article
Multiplex polymerase chain reaction with DNA pooling: a cost-effective strategy of genotyping rare blood types.
Transfusion medicine (Oxford, England) - 1 Feb 2013
He Y-L, Gao H-H, Ye L-Y, Guo Z-H, Wang P, Zhu Z-Y
Abstract excerpt
OBJECTIVES/AIMS: This work aims to develop a multiplex polymerase chain reaction combined with DNA pooling for mass screening for rare blood types. BACKGROUND: The differences in most blood group antigens are associated with single-nucleotide polymorphisms (SNPs), which are used in detecting blood antigen expression at the molecular level. However, all existing sequence-specific primers polymerase chain reaction...
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