Article
Nucleocytoplasmic transport is enhanced concomitant with nuclear accumulation of epidermal growth factor (EGF) binding activity in both 3T3-1 and EGF receptor reconstituted NR-6 fibroblasts.
The Journal of cell biology - 1 Mar 1990
Jiang L W, Schindler M
Abstract excerpt
Measurements of nucleocytoplasmic transport of fluorescent-labeled macromolecules were performed in both an EGF-nonresponsive mutant fibroblast line (3T3-NR6) and in the same cell line reconstituted with active EGF receptors derived from rat hepatic membrane fraction. Immunolocalization studies o...
Topics
- Animals
- Biological Transport
- Cell Membrane
- Cell Nucleus
- Cells, Cultured
- Chloroquine
- Cytoplasm
- Epidermal Growth Factor
- ErbB Receptors
- Fibroblasts
- Kinetics
- Leupeptins
- Mice
- Mutation
- Spectrometry, Fluorescence
- Thymidine