Article
DNA polymerase α (swi7) and the flap endonuclease Fen1 (rad2) act together in the S-phase alkylation damage response in S. pombe.
PloS one - 1 Jan 2012
Koulintchenko Milana, Vengrova Sonya, Eydmann Trevor, Arumugam Prakash, Dalgaard Jacob Z
Abstract excerpt
Polymerase α is an essential enzyme mainly mediating Okazaki fragment synthesis during lagging strand replication. A specific point mutation in Schizosaccharomyces pombe polymerase α named swi7-1, abolishes imprinting required for mating-type switching. Here we investigate whether this mutation confers any genome-wide defects. We show that the swi7-1 mutation renders cells hypersensitive to the DNA damaging...
Topics
- Alkylating Agents
- Cell Cycle Proteins
- DNA Damage
- DNA Polymerase I
- DNA Repair
- DNA-Binding Proteins
- Endodeoxyribonucleases
- Genomic Instability
- Hydroxyurea
- Methyl Methanesulfonate
- Mutation
- Nuclear Proteins
- S Phase