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Article

Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis.

BMC bioinformatics - 11 Oct 2012

Wang Chenghua, Huang Ribo, He Bingfang, Du Qishi

Abstract excerpt

BACKGROUND: The generation of focused mutant libraries at hotspot residues is an important strategy in directed protein evolution. Existing methods, such as combinatorial active site testing and residual coupling analysis, depend primarily on the evolutionary conserved information to find the hot...

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Identifiers

PMID: 23057711
DOI: 10.1186/1471-2105-13-263
PMCID: PMC3478181

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23057711-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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