Article
Protein Kinase C recognition sites in the cytoplasmic domain of Endothelin Converting Enzyme-1c.
Biochemical and biophysical research communications - 26 Oct 2012
Kuruppu Sanjaya, Tochon-Danguy Nathalie, Smith A Ian
Abstract excerpt
Endothelin Converting Enzyme-1 (ECE-1) is essential for the production of the potent vasoconstrictor Endothelin-1 (ET-1). The activation of Protein Kinase C (PKC) by phorbol 12-myristate 13-acetate (PMA) can increase ECE-1 phosphorylation, which in turn promotes ECE-1c trafficking to the cell surface where ET-1 production occurs. This study has identified the specific residues in the N-terminal cytoplasmic tail...
Topics
- Animals
- Aspartic Acid Endopeptidases
- CHO Cells
- COS Cells
- Chlorocebus aethiops
- Cricetinae
- Cytoplasm
- Endothelin-Converting Enzymes
- Gene Knockout Techniques
- HEK293 Cells
- Humans
- Metalloendopeptidases
- Mice
- Mutation
- Phosphorylation
- Protein Kinase C
- Protein Structure, Tertiary
- Tetradecanoylphorbol Acetate