Article
Activity-based protein profiling of the Escherichia coli GlpG rhomboid protein delineates the catalytic core.
Biochemistry - 2 Oct 2012
Sherratt Allison R, Blais David R, Ghasriani Houman, Pezacki John Paul, Goto Natalie K
Abstract excerpt
Rhomboid proteins comprise the largest class of intramembrane protease known, being conserved from bacteria to humans. The functional status of these proteases is typically assessed through direct or indirect detection of peptide cleavage products. Although these assays can report on the ability of a rhomboid to catalyze peptide bond cleavage, differences in measured hydrolysis rates can reflect changes in the...
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