Article
Heterotropic and homotropic cooperativity by a drug-metabolising mutant of cytochrome P450 BM3.
Biochemical and biophysical research communications - 4 Aug 2006
van Vugt-Lussenburg Barbara M A, Damsten Micaela C, Maasdijk Dianne M, Vermeulen Nico P E, Commandeur Jan N M
Abstract excerpt
Recently, we described a triple mutant of the bacterial cytochrome P450 BM3 as the first mutant with affinity for drug-like compounds. In this paper, we show that this mutant, but not wild-type BM3, is able to metabolise testosterone and several drug-like molecules such as amodiaquine, dextromethorphan, acetaminophen, and 3,4-methylenedioxymethylamphetamine that are known substrates of human P450s. Interestingly,...
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