Article
Arginine residues in the C-terminal and their relationship with the analgesic activity of the toxin from the Chinese scorpion Buthus martensii Karsch (BmK AGP-SYPU1).
Applied biochemistry and biotechnology - 1 Sept 2012
Wang Yu, Song Yong-Bo, Yang Guang-Zhao, Cui Yong, Zhao Yong-Shan, Liu Yan-Feng, Ma Yan, Wu Chun-Fu, Zhang Jing-Hai
Abstract excerpt
In this study, we investigated the functional role of arginines in the C-terminal (65-67) of BmK AGP-SYPU1, an analgesic peptide from the Chinese scorpion Buthus martensii Karsch. Using site-directed mutagenesis, arginines at the C-terminal (65-66) were deleted or added to the C-terminal (67). The genes for three mutants of BmK AGP-SYPU1 were obtained by PCR. An analgesic activity assay was used to evaluate the...
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