Optimization of gene expression through divergent mutational paths.

Abstract excerpt

Adaptation under similar selective pressure often leads to comparable phenotypes. A longstanding question is whether such phenotypic repeatability entails similar (parallelism) or different genotypic changes (convergence). To better understand this, we characterized mutations that optimized expression of a plasmid-borne metabolic pathway during laboratory evolution of a bacterium. Expressing these pathway genes...

Topics

• Base Sequence
• Flow Cytometry
• Gene Expression Regulation, Bacterial
• Genetic Fitness
• Glutathione
• Haplotypes
• Metabolic Engineering
• Methanol
• Methylobacterium extorquens
• Molecular Sequence Data
• Mutation