A plasmid vector for an extreme thermophile, Thermus thermophilus.

doi:10.1016/0378-1097(90)90352-q

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Article

A plasmid vector for an extreme thermophile, Thermus thermophilus.

FEMS microbiology letters - 1 Oct 1990

Koyama Y, Arikawa Y, Furukawa K

Abstract excerpt

The host-vector system for an extreme thermophile, Thermus thermophilus HB27, was developed. The host strain has a mutation in tryptophan synthetase gene (trpB), and the mutation was determined to be a missense mutation by DNA sequence analysis. A Thermus-E. coli shuttle vector pYK109 was constru...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Genetic Vectors
Molecular Sequence Data
Mutation
Plasmids
Restriction Mapping
Thermus
Transformation, Bacterial
Tryptophan Synthase

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