Article
A plasmid vector for an extreme thermophile, Thermus thermophilus.
FEMS microbiology letters - 1 Oct 1990
Koyama Y, Arikawa Y, Furukawa K
Abstract excerpt
The host-vector system for an extreme thermophile, Thermus thermophilus HB27, was developed. The host strain has a mutation in tryptophan synthetase gene (trpB), and the mutation was determined to be a missense mutation by DNA sequence analysis. A Thermus-E. coli shuttle vector pYK109 was constructed. pYK109 consists of Thermus cryptic plasmid pTT8, tryptophan synthetase gene (trpB) of Thermus T2 and E. coli...
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