Article
Isolation and characterization of a restriction and modification deficient mutant of Brevibacterium lactofermentum.
FEMS microbiology letters - 1 Oct 1990
Bonnassie S, Oreglia J, Trautwetter A, Sicard A M
Abstract excerpt
In order to facilitate genetic engineering in amino-acid producing bacteria we have isolated two restriction-deficient Brevibacterium lactofermentum strains. They have been selected for their ability to obtain a high yield of plaques from CL31 phage which was grown on Corynebacterium lilium. These mutant strains do not restrict either phage DNA by transfection or DNA from the shuttle vector pBLA extracted from...
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