Article
Mutation in the lysA gene impairs the symbiotic properties of Mesorhizobium ciceri.
Archives of microbiology - 1 Jan 2010
Das Subrata K, Gautam Uma Shankar, Sandhu Kiran V, Bandyopadhyay Saumya, Chakrabartty Pran K, Singh Aqbal
Abstract excerpt
A Tn5-induced mutant of Mesorhizobium ciceri, TL28, requiring the amino acid lysine for growth on minimal medium was isolated and characterized. The Tn5 insertion in the mutant strain TL28 was located on a 6.8-kb EcoRI fragment of the chromosomal DNA. Complementation analysis with cloned DNA indicated that 1.269 kb of DNA of the 6.8-kb EcoRI fragment restored the wild-type phenotype of the lysine-requiring...
Topics
- Alphaproteobacteria
- Amino Acid Sequence
- Bacterial Proteins
- Carboxy-Lyases
- Cicer
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Dicarboxylic Acid Transporters
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genome, Bacterial
- Molecular Sequence Data