Article
Human UDP-glucuronosyltransferase expression in insect cells: ratio of active to inactive recombinant proteins and the effects of a C-terminal his-tag on glucuronidation kinetics.
Drug metabolism and disposition: the biological fate of chemicals - 1 Oct 2012
Zhang Hongbo, Patana Anne-Sisko, Mackenzie Peter I, Ikushiro Shinichi, Goldman Adrian, Finel Moshe
Abstract excerpt
Many laboratories use recombinant UDP-glucuronosyltransferases (UGTs), expressed in baculovirus-infected insect cells, for drug glucuronidation studies. We have infected Sf9 insect cells with increasing amounts of recombinant baculovirus, encoding either UGT1A9 or UGT2B7, and measured both glucuronidation activity and immunodetectable UGT in the resulting cell homogenates. The correlation between glucuronidation...
Topics
- Animals
- Baculoviridae
- Blotting, Western
- Catalytic Domain
- Enzyme Activation
- Glucuronides
- Glucuronosyltransferase
- Histidine
- Humans
- Kinetics
- Models, Biological
- Mutation
- Recombinant Fusion Proteins