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Article

Alanine scanning mutagenesis of a high-affinity nitrate transporter highlights the requirement for glycine and asparagine residues in the two nitrate signature motifs.

The Biochemical journal - 1 Oct 2012

Unkles Shiela E, Karabika Eugenia, Symington Vicki F, Cecile Jennifer L, Rouch Duncan A, Akhtar Naureen, Cromer Brett A, Kinghorn James R

Abstract excerpt

Common to all of the nitrate nitrite porter family are two conserved motifs in transmembrane helices 5 and 11 termed NS (nitrate signature) 1 and NS2. Although perfectly conserved substrate-interacting arginine residues have been described in transmembrane helices 2 and 8, the role of NSs has not been investigated. In the present study, a combination of structural modelling of NrtA (nitrate transporter from...

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Identifiers

PMID: 22738143
DOI: 10.1042/BJ20120631

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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