An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance.

Abstract excerpt

Amt proteins constitute a class of ubiquitous integral membrane proteins that mediate movement of ammonium across cell membranes. They are homotrimers, in which each subunit contains a narrow pore through which substrate transport occurs. Two conserved histidine residues in the pore have been proposed to be necessary for ammonia conductance. By analyzing 14 engineered polar and non-polar variants of these...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Ammonia
• Share to Cation Transport Proteins