Article
Vital dye reaction and granule localization in periplasm of Escherichia coli.
PloS one - 1 Jan 2012
Ping Liyan, Mavridou Despoina A I, Emberly Eldon, Westermann Martin, Ferguson Stuart J
Abstract excerpt
BACKGROUND: Tetrazolium salts are widely used in biology as indicators of metabolic activity - hence termed vital dyes - but their reduction site is still debated despite decades of intensive research. The prototype, 2,3,5- triphenyl tetrazolium chloride, which was first synthesized a century ago, often generates a single formazan granule at the old pole of Escherichia coli cells after reduction. So far, no...
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