Article
Cell population heterogeneity in expression of a gene-switching network with fluorescent markers of different half-lives.
Journal of biotechnology - 1 Feb 2007
Portle Stephanie, Causey Thomas B, Wolf Kim, Bennett George N, San Ka-Yiu, Mantzaris Nikos
Abstract excerpt
We studied the distribution of expression levels amongst the cells of an Escherichia coli population carrying a gene-switching network, known as the genetic toggle. We employed two green fluorescent protein (GFP) reporter proteins with different half-lives and characterized the effect of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducer concentration on fluorescence distribution characteristics. Our flow...
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