Article
Methods for diagnostic HLA typing in disease association and drug hypersensitivity.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Varney Michael D, Castley Alison S L, Haimila Katri, Saavalainen Päivi
Abstract excerpt
This chapter describes the application of diagnostic HLA typing for disease association and five methods used for specific HLA genotypes. The methods utilise a combination of polymerase chain reaction (PCR) amplification to detect sequence polymorphism by the presence or absence of amplification, nucleotide sequencing of the PCR product, and hybridisation of the PCR product with labelled probes. The probes are...
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