Bioassay-directed fractionation of 1-nitropyrene metabolites: generation of mutagrams by coupling reverse-phase HPLC with microsuspension mutagenicity assays.

doi:10.1093/mutage/5.5.481

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Article

Bioassay-directed fractionation of 1-nitropyrene metabolites: generation of mutagrams by coupling reverse-phase HPLC with microsuspension mutagenicity assays.

Mutagenesis - 1 Sept 1990

Lewtas J, King L C, Williams K, Ball L M, DeMarini D M

Abstract excerpt

We have performed bioassay-directed fractionation of a model complex mixture (rabbit lung S9-generated metabolites of 14C-radiolabeled 1-nitropyrene) by assaying reverse-phase HPLC fractions using two microsuspension mutagenicity assays. A forward-mutation assay measuring mutation at the gpt locu...

Topics

Animals
Biological Assay
Chromatography, High Pressure Liquid
Lung
Male
Mutagenicity Tests
Mutation
Pyrenes
Rabbits
Salmonella typhimurium

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