Article
Mutation spectrum of cigarette smoke condensate in Salmonella: comparison to mutations in smoking-associated tumors.
Carcinogenesis - 1 Oct 1995
DeMarini D M, Shelton M L, Levine J G
Abstract excerpt
We used colony probe hybridization and polymerase chain reaction/DNA sequence analysis to determine the mutations in approximately 1600 revertants of Salmonella induced by cigarette smoke condensate (CSC) in the presence of S9. CSC induced approximately 80% GC-->TA transversions and approximately 20% GC-->AT transitions at the base-substitution allele (hisG46) in strain TA100. This spectrum was similar to those...
Topics
- Alleles
- Animals
- Base Sequence
- Benzo(a)pyrene
- Biotransformation
- DNA, Bacterial
- DNA, Neoplasm
- Humans
- Microsomes, Liver
- Molecular Sequence Data
- Mutagenesis
- Mutagenicity Tests