Article
A method for killer-cell immunoglobulin-like receptor (KIR) 3DL1/3DS1 genotyping using DNA recovered from frozen plasma.
Journal of immunological methods - 31 May 2013
Shahid Aniqa, Chopera Denis R, Martin Eric, Penney Kali A, Milloy M-J, Brumme Zabrina L
Abstract excerpt
We describe a reliable and semi-automated method for killer-cell immunoglobulin-like receptor (KIR) 3DL1/S1 genotyping using DNA recovered from frozen plasma. The primers and protocol were first validated using two independent genomic DNA reference panels. To confirm the approach using plasma-derived DNA, total nucleic acids were extracted from 69 paired frozen PBMC and plasma specimens representing all common...
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