Article
RNA splicing in Chlamydomonas chloroplasts. Self-splicing of 23 S preRNA.
The Journal of biological chemistry - 5 Dec 1990
Herrin D L, Chen Y F, Schmidt G W
Abstract excerpt
The 23 S rRNA gene of the Chlamydomonas reinhardtii chloroplast contains an 888-base pair intron with structural features characteristic of Group I introns. The nuclear, chloroplast ribosome-deficient mutant of C. reinhardtii, ac20, overaccumulates a approximately 3.6-kilobase unspliced 23 S preRNA compared to wild-type cells. We have used [alpha-32P]GTP labeling of total RNA preparations from ac20 to rapidly...
Topics
- Base Sequence
- Chlamydomonas
- Chloroplasts
- DNA Probes
- Introns
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- RNA Precursors
- RNA Splicing
- RNA, Ribosomal, 23S
- Restriction Mapping