Article
Correct in vivo RNA splicing of a mitochondrial intron in algal chloroplasts.
Nucleic acids research - 11 Aug 1994
Herdenberger F, Holländer V, Kück U
Abstract excerpt
The self-splicing group II intron (rl1) from Scenedesmus obliquus mitochondria together with its 6 bp intron binding site (IBS1) were inserted in the correct and inverse orientation into the chloroplast tscA gene from C.reinhardtii. Precursor RNA derived from the chimeric tscA-rl1 gene can be used to demonstrate in vitro self-splicing of the rl1 intron RNA. Using the particle bombardment technique, the tscA-rl1...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Chlamydomonas reinhardtii
- Chlorophyta
- Chloroplasts
- Gene Transfer Techniques
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization