Article
Raa4 is a trans-splicing factor that specifically binds chloroplast tscA intron RNA.
The Plant journal : for cell and molecular biology - 1 Feb 2012
Glanz Stephanie, Jacobs Jessica, Kock Vera, Mishra Arti, Kück Ulrich
Abstract excerpt
During trans-splicing of discontinuous organellar introns, independently transcribed coding sequences are joined together to generate a continuous mRNA. The chloroplast psaA gene from Chlamydomonas reinhardtii encoding the P(700) core protein of photosystem I (PSI) is split into three exons and two group IIB introns, which are both spliced in trans. Using forward genetics, we isolated a novel PSI mutant, raa4,...
Topics
- Chlamydomonas reinhardtii
- Chloroplast Proteins
- Genetic Complementation Test
- Introns
- Mutation
- Nucleic Acid Conformation
- Photosystem I Protein Complex
- Plant Proteins
- RNA, Chloroplast
- RNA, Plant
- RNA-Binding Proteins
- Trans-Splicing