Article
Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii.
Open biology - 1 Mar 2018
Lin Huawen, Zhang Zhengyan, Iomini Carlo, Dutcher Susan K
Abstract excerpt
Intraflagellar transport moves proteins in and out of flagella/cilia and it is essential for the assembly of these organelles. Using whole-genome sequencing, we identified splice site mutations in two IFT genes, IFT81 (fla9) and IFT121 (ift121-2), which lead to flagellar assembly defects in the unicellular green alga Chlamydomonas reinhardtii The splicing defects in these ift mutants are partially corrected by...
Topics
- Algal Proteins
- Chlamydomonas reinhardtii
- Frameshift Mutation
- Mutation
- Nonsense Mediated mRNA Decay
- RNA Splice Sites
- RNA Splicing Factors
- Sequence Deletion
- Whole Genome Sequencing