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Article

Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat model

5 Apr 2012

Abstract excerpt

Obesity is a complex pathology with interacting and confounding causes due to the environment, hormonal signaling patterns, and genetic predisposition. At present, the Zucker rat is an eligible genetic model for research on obesity and metabolic syndrome, allowing scrutiny of gene expression profiles. Real-time PCR is the benchmark method for measuring mRNA expressions, but the accuracy and reproducibility of its...

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PMID: 22493144
DOI: 10.1530/jme-12-0024

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