Article

A functional genomic approach to identify reference genes for human pancreatic beta cell real-time quantitative RT-PCR analysis

2021-04-14

Abstract excerpt

Exposure of human pancreatic beta cells to pro-inflammatory cytokines or metabolic stressors is used to model events related to type 1 and type 2 diabetes, respectively. Quantitative real-time PCR is commonly used to quantify changes in gene expression. The selection of the most adequate reference gene(s) for gene expression normalization is an important pre-requisite to obtain accurate and reliable results. There...

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Literature Corpus work: a5c75f30-328b-506a-885b-afeda054c55c
DOI: 10.1101/2021.04.14.439798

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