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Article

Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes.

PloS one - 13 Dec 2010

Ferguson Bradley S, Nam Heesun, Hopkins Robin G, Morrison Ron F

Abstract excerpt

BACKGROUND: With the current rise in obesity-related morbidities, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used method for assessment of genes expressed and regulated by adipocytes. In order to measure accurate changes in relative gene e...

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Identifiers

PMID: 21179435
DOI: 10.1371/journal.pone.0015208
PMCID: PMC3001470

Genes

GAPDH

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-21179435-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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