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Article

Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes.

PloS one - 13 Dec 2010

Ferguson Bradley S, Nam Heesun, Hopkins Robin G, Morrison Ron F

Abstract excerpt

BACKGROUND: With the current rise in obesity-related morbidities, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used method for assessment of genes expressed and regulated by adipocytes. In order to measure accurate changes in relative gene expression and monitor intersample variability, normalization to endogenous control genes that do not change in relative...

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Identifiers

PMID: 21179435
DOI: 10.1371/journal.pone.0015208
PMCID: PMC3001470

Genes

GAPDH

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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