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Article

Evaluation of the stability of standard reference genes of adipose-derived mesenchymal stem cells during in vitro proliferation and differentiation.

Molecular biology reports - 1 Mar 2020

Ayanoğlu Fatma Betül, Elçin Ayşe Eser, Elçin Yaşar Murat

Abstract excerpt

Mesenchymal stem cells (MSCs) from a variety of sources are being used in pre-clinical and clinical studies. The choice of optimal source for treatment of diseases requires quantitative evaluation of self-renewal, proliferation and differentiation potencies of MSCs. For this purpose, quantitative real-time polymerase chain reaction (qRT-PCR) technique is used to determine the expression of genes. qRT-PCR requires...

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PMID: 32065324
DOI: 10.1007/s11033-020-05311-y

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