Article
Importance of exposed aromatic residues in chitinase B from Serratia marcescens 2170 for crystalline chitin hydrolysis.
Journal of biochemistry - 1 Aug 2004
Katouno Fuminori, Taguchi Masashi, Sakurai Kengo, Uchiyama Taku, Nikaidou Naoki, Nonaka Takamasa, Sugiyama Junji, Watanabe Takeshi
Abstract excerpt
Chitinase B (ChiB) of S. marcescens has five exposed aromatic residues linearly aligned toward the catalytic cleft, Tyr481 and Trp479 in the C-terminal domain, and Trp252, Tyr240 and Phe190 in the catalytic domain. To determine the contribution of these residues to the hydrolysis of crystalline beta-chitin, site-directed mutagenesis, to replace them by alanine, was carried out. The Y481A, W479A, W252A, and Y240A...
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