Article
Examination of the role of tyrosine-174 in the catalytic mechanism of the Arabidopsis thaliana chitinase: comparison of variant chitinases generated by site-directed mutagenesis and expressed in insect cells using baculovirus vectors.
Archives of biochemistry and biophysics - 1 Jan 1993
Verburg J G, Rangwala S H, Samac D A, Luckow V A, Huynh Q K
Abstract excerpt
Using the catalytic mechanism of lysozyme as a paradigm for the mechanism of other enzymes that catalyze the hydrolysis of beta-1,4-glycosidic linkages, including chitinase, we have examined the effect of chemical modification with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) on the react...
Topics
- Amino Acid Sequence
- Animals
- Arabidopsis
- Baculoviridae
- Base Sequence
- Binding Sites
- Cell Line
- Chitinases
- Genetic Variation
- Genetic Vectors
- Insecta
- Kinetics
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Site-Directed