Article
The CDC4 gene product is associated with the yeast nuclear skeleton.
Biochemical and biophysical research communications - 15 Nov 1990
Choi W J, Clark M W, Chen J X, Jong A Y
Abstract excerpt
The CDC4 gene product of Saccharomyces cerevisiae is required at the late G1/S phase boundary of the cell cycle. In an attempt to better understand the function of CDC4, we performed experiments to localize this protein in the yeast cell. Using antisera, directed against a TrpE-CDC4 fusion protein, to analyze immuno-blots of different subcellular fractions from yeast, we demonstrated that the CDC4 gene product...
Topics
- Cell Cycle
- Cell Nucleus
- Cloning, Molecular
- Fungal Proteins
- Microscopy, Fluorescence
- Mutation
- Plasmids
- Saccharomyces cerevisiae