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Article

Concurrent serotyping and genotyping of pneumococci by use of PCR and electrospray ionization mass spectrometry.

Journal of clinical microbiology - 1 Jun 2012

Massire Christian, Gertz Robert E, Svoboda Pavel, Levert Keith, Reed Matthew S, Pohl Jan, Kreft Rachel, Li Feng, White Neill, Ranken Ray, Blyn Larry B, Ecker David J, Sampath Rangarajan, Beall Bernard

Abstract excerpt

A pneumococcal serotyping/genotyping system (PSGS) was developed based upon targeted PCR, followed by electrospray ionization mass spectrometry and amplicon base composition analysis. Eight multiplex PCRs, 32 targeting serotype-determining capsular biosynthetic loci, and 8 targeting multilocus sequence typing (MLST) loci were employed for each of 229 highly diverse Streptococcus pneumoniae isolates. The most...

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Identifiers

PMID: 22442313
DOI: 10.1128/JCM.06735-11
PMCID: PMC3372165

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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