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Article

Rapid molecular genotyping and clonal complex assignment of Staphylococcus aureus isolates by PCR coupled to electrospray ionization-mass spectrometry.

Journal of clinical microbiology - 1 Jun 2009

Hall Thomas A, Sampath Rangarajan, Blyn Lawrence B, Ranken Raymond, Ivy Cristina, Melton Rachael, Matthews Heather, White Neill, Li Feng, Harpin Vanessa, Ecker David J, McDougal Linda K, Limbago Brandi, Ross Tracy, Wolk Donna M, Wysocki Vicki, Carroll Karen C

Abstract excerpt

We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (MLST). The method was used to identify the clonal complex and USA type of each isolate and is suitable for use in a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19297593
DOI: 10.1128/JCM.02175-08
PMCID: PMC2691079

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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