Article
Multilocus sequence typing of Streptococcus pneumoniae by use of mass spectrometry.
Journal of clinical microbiology - 1 Nov 2011
Dunne Eileen M, Ong Eng Kok, Moser Ralf J, Siba Peter M, Phuanukoonnon Suparat, Greenhill Andrew R, Robins-Browne Roy M, Mulholland E Kim, Satzke Catherine
Abstract excerpt
Multilocus sequence typing (MLST) is an important tool for the global surveillance of bacterial pathogens that is performed by comparing the sequences of designated housekeeping genes. We developed and tested a novel mass spectrometry-based method for MLST of Streptococcus pneumoniae. PCR amplicons were subjected to in vitro transcription and base-specific cleavage, followed by analysis of the resultant fragments...
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