Article
Molecular genotyping of microbes by multilocus PCR and mass spectrometry: a new tool for hospital infection control and public health surveillance.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Ecker David J, Massire Christian, Blyn Lawrence B, Hofstadler Steven A, Hannis James C, Eshoo Mark W, Hall Thomas A, Sampath Rangarajan
Abstract excerpt
We describe a new technology for the molecular genotyping of microbes using a platform known commercially as the Ibis T5000. The technology couples multilocus polymerase chain reaction (PCR) to electrospray ionization/mass spectrometry (PCR/ESI-MS) and was developed to provide rapid, high-throughput, and precise digital analysis of either isolated colonies or original patient specimens on a platform suitable for...
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