Article
Development of a bacterial artificial chromosome (BAC) recombineering procedure using galK-untranslated region (UTR) for the mutation of diploid genes.
Journal of virological methods - 1 Jun 2012
Dai Gan, Kim Seongman, O'Callaghan Dennis J, Kim Seong K
Abstract excerpt
Bacterial artificial chromosome (BAC) recombineering using galK selection allows DNA cloned in Escherichia coli to be modified without introducing an unwanted selectable marker at the modification site. Genomes of some herpesviruses have a pair of inverted repeat sequences that makes it very diff...
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