Article
Synthesis and secretory expression of hybrid antimicrobial peptide CecA-mag and its mutants in Pichia pastoris.
Experimental biology and medicine (Maywood, N.J.) - 1 Mar 2012
Wang Xiuqing, Zhu Mingxing, Zhang Aijun, Yang Fengqin, Chen Puyan
Abstract excerpt
The hybrid peptide CA(1-7)-M(2-12) gene was designed according to the N-terminal 1-7 amino acid sequence of the antimicrobial peptide cecropin A (CA) and the N-terminal 2-12 amino acid sequence of maganin (M) and synthesized using Pichia pastoris preferred codons. The gene was cloned into pPICZαA and transformed into the P. pastoris recipient bacterium SMD1168, regulated by the alcohol oxidase (AOX). Expression...
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