Article
[High expression of antimicrobial peptide Cecropin AD in Escherichia coli by fusion with EDDIE].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Aug 2009
Zhang Zhen, Ke Tao, Zhou Yuling, Ma Xiangdong, Ma Lixin
Abstract excerpt
In this study, we efficiently expressed the active antimicrobial peptide (CAD), which fused with the site-mutated coat protein (EDDIE) of the classical swine fever virus, in Escherichia coli. First, we obtained the e-cad fusion gene from the CAD gene and the EDDIE gene using overlapping PCR. Then...
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